Maanei e wehewehe mākou i ka hana ʻana i ka electrophoresis gel agarose e kā mākou mea noiʻi ma ka lab o Liuyi Biotech.
Ma mua o ka hoʻokolohua, pono mākou e nānā i nā mea hana, reagents, a me nā mea hoʻokolohua ʻē aʻe a me nā mea hana e pono ai mākou.
Hoʻomākaukau i nā mea hoʻokolohua a me nā mea hana
ʻO nā mea hana no ka electrophoresis gel agarose
ʻO ke kelepona electrophoresis horizontal (tank/chamber), ka lako mana electrophoresis waena a me ka haʻahaʻa, kiʻi gel a me ka ʻōnaehana loiloi.
Hāʻawi ʻo Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) i nā hiʻohiʻona like ʻole o ka cell electrophoresis horizontal (tank/chamber) a me ka lako mana a me ka ʻōnaehana palapala gel.ʻO nā huahana DYCP-31 series no ka electrophoresis, a ʻo nā huahana DYY series ka lako mana electrophoresis.ʻO nā huahana WD-9413 ka ʻōnaehana palapala gel.Mai ka liʻiliʻi gel liʻiliʻi 60 × 60mm i ka gel nui 250 × 250mm, hiki iā mākou ke hoʻokō i kāu mau koi like ʻole no ka nui gel.ʻO ke kumu hoʻohālikeDYCP-32Chiki ke hana i ka gel a hiki i ka nui 250 × 250mm.ʻO kaDYY-6CʻO kā mākou lako mana.Kākoʻo ia i kahi puka o 400V, 400mA, 240W, ʻo ia kā mākou huahana maʻamau i hoʻohana ʻia e kā mākou mea kūʻai. WD-9413BHoʻohana ʻia ʻo Gel Image & Analysis System no ka nānā ʻana a me ka noiʻi ʻana no ka gel, nā kiʻiʻoniʻoni, a me nā blots ma hope o ka hoʻokolohua electrophoresis.He mea maʻamau ia me ke kumu kukui ultraviolet no ka nānā ʻana a me ke kiʻi ʻana i nā gels i hoʻopaʻa ʻia me nā mea hoʻonaninani fluorescent e like me ka ethidium bromide, a me ke kumu kukui keʻokeʻo no ka nānā ʻana a me ke kiʻi ʻana i nā gels i hoʻopaʻa ʻia me nā kala e like me ka coomassie brilliant blue.
ʻO nā reagents no ka electrophoresis gel agarose
1. ʻO ke kikowaena: agarose gel
2. ʻO ka pale: TAE (Tris-acetate, EDTA, glacial acetic acid) a me TBE (Tris-borate, EDTA, boric acid).
3. ʻO ka hoʻouka ʻana: 6×DNA Loading Buffer (Special for DNA sample: EDAT, glycerine, Xylene cyanol and bromophenol blue)
Ka Waiole
ʻO ke kala ʻulaʻula e like me EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
ʻO kaMea hoopau
ʻO nā ʻōlelo aʻoaʻo pipette sterilization (10μL), nā ʻōlelo aʻoaʻo pipette (200μL), nā ʻōlelo pipette (1000μL), 200μL\500μL\1.5ml centrifuge tube.
Hoailona DNA
Hoʻomākaukau ʻia e like me ke kaumaha molekala e hoʻāʻo ʻia.
Ke holo nei ka Gel a nānā
ʻO ka mea mua, pono mākou e hoʻomākaukau i ka gel agarose.E hilinaʻi ka ʻike o ka agarose i loko o kahi gel i ka nui o nā ʻāpana DNA e hoʻokaʻawale ʻia, me ka hapa nui o nā gels ma waena o 0.5% -2%.Lawe i kā mākouDYCP-31DNno ka laʻana, Hiki iā ʻoe ke hoʻolei i ka gel liʻiliʻi, gel ākea , gel lōʻihi a me ka gel square e like me nā koi o ka hoʻokolohua.E koho i ka pahu gel e pono ai, a hoʻokomo i ka lei, a laila ninini i ka gel agarose wela i loko o ka mea hoʻolei gel.
A laila, ma hope o ka paʻa ʻana o ka gel, e lawe pono i ka lehu me ka mālie, a laila e hoʻokomo i ka pā gel i loko o ka pahu pahu.E ninini i ka wai hoʻoheheʻe i loko o ka pahu hoʻopaʻa a hoʻokomo i ka gel a pau i loko o ka pahu.E hoʻouka i nā laʻana i loko o nā pūnāwai me kahi pipette maʻamau.Hoʻohui i kaDYCP-31DNme ka lako electrophoresis pono, a hoonoho i na palena e holo i ka gel.
E ʻike i kāu mau ʻāpana DNA i kahi transilluminator UV.
AMa hope o ka holo ʻana i ka gel, hiki iā ʻoe ke hoʻohana i kā mākou kiʻi gel & ʻōnaehana ʻōnaehanaWD-9413Be nānā, kālailai a paʻi kiʻi no ka gel.Hāʻawi pū ʻo Liuyi Biotech i ka UV transilluminator (UV Analyzer) e nānā i ka gel.Loaʻa iā mākou nā ʻano pahu ʻeleʻele UV transilluminator (UV Analyzer).WD-9403A, 9403C, WD-9403F, Ke kumu hoʻohālike UV transilluminator (UV Analyzer).WD-9403Ba paʻa lima UV transilluminator (UV Analyzer)WD-9403Eno kou koho ana.
He kiʻi o ka gel post electrophoresis
Ua ʻoi aku ka nui o ka brand Liuyi ma mua o 50 mau makahiki o ka mōʻaukala ma Kina a hiki i ka hui ke hāʻawi i nā huahana kūpaʻa a kiʻekiʻe a puni ka honua.Ma o nā makahiki o ka hoʻomohala ʻana, kūpono ia i kāu koho!
No ka ʻike hou aku e pili ana iā mākou, e ʻoluʻolu e kelepona mai iā mākou ma ka leka uila[palapala leka uila], [palapala leka uila].
Ka manawa hoʻouna: Apr-29-2022