Agarosegel electrophoresis
ʻO Agarose gel electrophoresis kahi ʻano o ka electrophoresis gel i hoʻohana ʻia i ka biochemistry, molecular biology, genetics, a me ka kemika lapaʻau e hoʻokaʻawale i kahi heluna hui o nā macromolecules e like me DNA a i ʻole.RNA. Loaʻa ʻia kēia ma ka hoʻoneʻe ʻana i nā molekele nucleic acid i hoʻopiʻi maikaʻi ʻia ma o kahi matrix agarose meelectrophoresis.
Nā waiwai o ka gel agarose
ʻO ka Agarose gel kahi matrix ʻekolu-dimensional i hoʻokumu ʻia i nā molekole helical agarose i loko o nā pūʻulu supercoiled i hoʻohui ʻia i loko o nā hale ʻekolu-dimensional me nā kahawai a me nā pores e hiki ai i nā biomolecules ke hele. ma ka hoʻomehana ʻana i ka wai.ʻOkoʻa ka mahana hoʻoheheʻe ʻana mai ka mahana gelling, ma muli o nā kumu, ʻo ka agarose gel ka mahana gelling o 35-42 °C a me kahi mahana hehee o 85-95 °C.Loaʻa pū ʻia nā agaroses haʻahaʻa haʻahaʻa a me ka haʻahaʻa gelling i hana ʻia ma o nā hoʻololi kemika.
ʻO nā pono a me nā pōʻino o ka electrophoresis gel agarose
ʻO ka pōmaikaʻi nui o ka ʻenehana electrophoresis gel agarose ʻo ia ka hiki ke maʻalahi a hiki ke hoʻihoʻi ʻia ka mole DNA i hoʻohana ʻia ma ke ʻano he laʻana me ka ʻole o ka pōʻino i ka hopena o ke kaʻina hana.No ka mea, ʻaʻole ka gel agarose't loaʻa i ka pūʻulu sulfate a me ka carboxyl, ʻaʻole ia e kumu i ka electroosmosis o ka agar.Me ke ʻano like ʻole a me ka nui o ka pore, maikaʻi ka gel agaroseka hoʻokaʻawale ʻana i nā molekala DNA nui, e like me enzyme complex, nucleic acid a me ka virus.ʻAʻole ʻo Agarose gel't e komo i ke kukui UV, no laila hiki ke hoʻopaʻa pololei ʻia a ma hope paha o ka gelling e lilo i kiʻiʻoniʻoni, hiki ke hoʻohuli ʻia hoʻi.
Aia kekahi mau hemahema o ka ʻenehana electrophoresis gel agarose.ʻAʻole maʻalahi ka haʻihaʻi ʻana o ka gel agarose, no laila ʻaʻole hiki ke haʻahaʻa loa ka haʻahaʻa, ʻoi aku ka nui o ka agarose.ʻO ka gel agarose He paʻakikī ke hoʻopakele no ka mea maʻalahi ke hoʻohaumia ʻia e ka bacteria, a pono e hoʻomākaukau ʻia ma mua o ka hoʻohana ʻana.Hoʻohālikelike i ka ʻenehana electrophoresis PAGE, ʻoi aku ka liʻiliʻi o ka sieve molecular, a emi iho ka wahi electrophoresis.
Kaupaeelectrophoresisʻōnaehana no ka gel agarose
Ma waho aʻe o ka agarose a me ka buffer, pono ka hoʻokolohua electrophoresis gel agarose i ka ʻōnaehana electrophoresis ākea a me nā haʻahaʻa haʻahaʻa.uila electrophoresis lako mana.Hiki iā Beijing Liuyi Biotechnology ke hāʻawi i ka ʻōnaehana electrophoresis ākea a electrophoresis mana lako, like modelDYCP-31BN, DYCP-31CN no ka meaʻōnaehana electrophoresis ākea aDYY-6C no ka lako mana.
Ke hilinaʻi nei ma nā ʻano gel like ʻolekoi ʻia e ka ʻO ka hoʻokolohua agrose gel electrophoresis, hiki ke hāʻawi ʻia nā nui like ʻole o ka ʻōnaehana electrophoresis e Beijing Liuyi Biotechnology.ʻO ke kumu hoʻohālike DYCP-31,such asDYCP-31BN, DYCP-31CN, DYCP-31DN, DYCP-31E, DYCP-32B, aDYCP-32CLoaʻa nā ʻano ʻokoʻa o ka pā gel, mai ka hoʻolei ʻana i ka gel liʻiliʻi i ka gel nui nui, hiki iā lākou ke hālāwai me kāu hoʻokolohuakoi.
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Ka manawa hoʻouna: Apr-16-2022