ʻO ka electrophoresis gel kekahi o nā ala nui i hoʻohana ʻia i ka biology molecular no ka nānā ʻana i ka DNA.Hoʻopili kēia ʻano hana i ka neʻe ʻana o nā ʻāpana DNA ma o kahi gel, kahi i hoʻokaʻawale ʻia ai ma muli o ka nui a i ʻole ke ʻano.Eia naʻe, ua loaʻa paha iā ʻoe kekahi hewa i ka wā o kāu mau hoʻokolohua electrophoresis, e like me nā kaula i hamo ʻia ma ka gel agarose, a i ʻole nā kaula ma ka gel?He aha ke kumu o kēia mau hewa?
Ua hōʻuluʻulu kā mākou mau ʻenehana i nā pilikia pilikia ma aneʻi no kāu kuhikuhi.
1. ʻO nā kaula i hamo ʻia ma ka gel agarose
●Ua hoʻohaʻahaʻa ʻia ka DNA.E pale i ka hoʻohaumia ʻana o ka nuclease.
● ʻAʻole hou ka pale electrophoresis.Ma hope o ka hoʻohana pinepine ʻana i ka electrophoresis buffer, emi ka ikaika o ka ionic, a piʻi ka pH waiwai, no laila nāwaliwali ka hiki ke hoʻopaʻa, e pili ana i ka hopena electrophoresis.Manaʻo ʻia e hoʻololi pinepine i ka pale electrophoresis.
● Ua hoʻohana ʻia nā kūlana electrophoresis kūpono ʻole.Mai ʻae i ka uila e ʻoi aku ma mua o 20 V/cm, a mālama i kahi mahana ma lalo o 30 ° C i ka wā electrophoresis.No ka nui o ka DNA strand electrophoresis, pono ka mahana ma lalo o 15° C.
● Nui loa ka DNA i hoʻouka ʻia ma ka gel.E ho'ēmi i ka nui o ka DNA.
● Nui loa ka paʻakai ma ka DNA.E hoʻohana i ka ua ethanol no ka wehe ʻana i nā paʻakai keu ma mua.
● Ua haumia ka DNA me ka protein.E hoʻohana i nā unuhi phenol e wehe i ka protein ma mua.
● Ua denatured ka DNA.Mai wela ma mua o ka electrophoresis.E hoʻoheheʻe i ka DNA i loko o ka pale me 20 mM NaCl.
2. Anomalies DNA band migration
● Hoʻihoʻi hou i ka pūnaewele COS o ka ʻāpana λHind III.E hoʻomehana i ka DNA no 5 mau minuke ma lalo o 65 ° C ma mua o ka electrophoresis, a laila e hoʻomaha iā ia ma luna o ka hau no 5 mau minuke.
● Ua hoʻohana ʻia nā kūlana electrophoresis kūpono ʻole.Mai ʻae i ka uila e ʻoi aku ma mua o 20 V/cm, a mālama i kahi mahana ma lalo o 30 ° C i ka wā electrophoresis.E hōʻoia i ka loaʻa ʻana o ka mana hoʻopaʻa electrophoresis.
● Ua denatured ka DNA.Mai wela ma mua o ka electrophoresis.E hoʻoheheʻe i ka DNA i loko o ka pale me 20 mM NaCl.
3. Maule a ʻaʻole ʻole nā kaula DNA ma ka gel agarose
● ʻAʻole lawa ka nui a i ʻole ka pae ʻana o ka DNA i hoʻouka ʻia ma ka gel.E hoʻonui i ka nui o ka DNA.ʻOi aku ka maʻalahi o ka polyacrylamide gel electrophoresis ma mua o ka electrophoresis agarose, a hiki ke hoʻemi kūpono ʻia ka hoʻouka ʻana.
● Ua hoʻohaʻahaʻa ʻia ka DNA.E pale i ka hoʻohaumia ʻana o ka nuclease.
● Hoʻopili ʻia ka DNA mai ka gel.Electrophorese i ka gel no ka manawa liʻiliʻi, e hoʻohana i ka volta haʻahaʻa, a i ʻole e hoʻohana i kahi gel kiʻekiʻe.
● Ua hoʻohana ʻia ke kumu kukui W kūpono ʻole no ka ʻike ʻana i ka DNA i hoʻopaʻa ʻia i ka ethidium bromide.E hoʻohana i ka nalu pōkole (254 nm) W kukui no ka ʻoi aku ka naʻauao.
4. Nalo nā kaula DNA
●ʻO ka DNA liʻiliʻi liʻiliʻi i electrophorese mai ka gel.Electrophorese i ka gel no ka manawa liʻiliʻi, e hoʻohana i ka volta haʻahaʻa, a i ʻole e hoʻohana i kahi gel kiʻekiʻe.
● Paʻakikī ke hoʻokaʻawale i nā kaula DNA o nā molekala like.E hoʻonui i ka manawa electrophoresis, a nānā i ka ʻikeo ka gel e hōʻoia pololei i ka pakeneka gel e hoʻohana ʻia.
● Ua denatured ka DNA.Mai wela ma mua o ka electrophoresis.E hoʻoheheʻe i ka DNA i loko o ka pale me 20 mM NaCl.
● Nui nā kaula DNA, a ʻaʻole kūpono ka electrophoresis gel maʻamau.E noʻonoʻo i ka pulse gel electrophoresis.He aha nā pilikia ʻē aʻe i loaʻa iā ʻoe me ka electrophoresis gel agarose?E noiʻi hou aku mākou no nā alakaʻi i ka wā e hiki mai ana.
ʻO Beijing Liuyi biotechnology Co., Ltd (Liuyi Biotech) he hui kūikawā e kālele ana i nā huahana pili electrophoresis ma Kina.Hoʻomaka kāna moʻolelo i ka makahiki 1970 i ka wā ʻaʻole i komo ʻo Kina i ka hoʻoponopono a me ka wehe ʻana.Ma o ka ulu ʻana o nā makahiki, loaʻa iā Liuyi Bitotech kāna hōʻailona ponoʻī, i ʻike ʻia ʻo ka Liuyi Brand ma ka mākeke kūloko no nā huahana electrophoresis.
Loaʻa i ka brand Liuyi ma mua o 50 mau makahiki moʻolelo ma Kina a hiki i ka hui ke hāʻawi i nā huahana kūpaʻa a kiʻekiʻe a puni ka honua.Ma o ka ulu ʻana o nā makahiki, kūpono ia i kāu koho!
ʻO nā keena electrophoresis ākea (nā pahu / keʻena) o Liuyi Biotech he kūlana kiʻekiʻe me ka helehelena maikaʻi.Me nā ʻano nui o nā pā gel, hiki iā lākou ke hoʻokō i kāu mau koi hoʻokolohua like ʻole.Loaʻa iā mākou kā mākou hui ʻenehana a me ka hale hana.Mai ka hoʻolālāʻana i ka hanaʻana, nā mea maka i nā māhele nui, hiki iā mākou ke hoʻomalu i ke kaʻina hana holoʻokoʻa.ʻO ka moʻo DYCP 31 no ka DNA electrophoresis, he kumu hoʻohālikeDYCP-31BN, DYCP-31CN,DYCP-31DN, aDYCP-31E.ʻO nā ʻokoʻa ma waena o lākou he nui gel a me ke kumukūʻai.Hāʻawi mākou i nā nui piha o nā huahana no kā mākou mea kūʻai.ʻO ke kumu hoʻohālikeDYCP-32Chiki ke hana i ka gel nui loa 250mm * 250mm.
I kēia manawa, paipai mākou i kā mākou lako mana electrophoresisDYY-6C,DYY-6DaDYY-10Cno kā mākou mau pūnaewele electrophoresis (nā pahu / keʻena) DYCP-31 a me 32 series.
Inā makemake ʻoe i ka ʻike hou aku e pili ana i nā huahana, e ʻoluʻolu e kipa i kēia pūnaewele no ka loaʻa ʻana o nā mea hou aʻe, a e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou ma o ka leka uila e haʻi mai iā mākou i kāu makemake, a ʻike inā hiki iā mākou ke hāʻawi i nā hāʻina no ʻoe.
No ka ʻike hou aku e pili ana iā mākou, e ʻoluʻolu e kelepona mai iā mākou ma ka leka uila[palapala leka uila], [palapala leka uila].
Ka manawa hoʻouna: Mei-09-2022