ʻO ka Polymerase Chain Reaction (PCR) kahi ʻenehana biology molecular i hoʻohana ʻia e hoʻonui i nā ʻāpana DNA kikoʻī. Hiki ke noʻonoʻo ʻia he ʻano kūikawā o ka replication DNA ma waho o kahi mea ola. ʻO ka hiʻohiʻona nui o ka PCR ʻo kona hiki ke hoʻonui nui i ka nui o ka DNA.
ʻO ka nānā ʻana o kahi pōʻaiapili kaulahao Polymerase
Kiʻi mai Bioninja
He aha ke kaʻina hana paikikala wela ma PCR?
Aia ʻo PCR i ʻekolu mau ʻanuʻu kumu:
1. Denaturation of Template DNA: Hoʻomaʻamaʻa ʻia ka DNA template ma kahi o 93°C no kekahi lōʻihi, e hoʻokaʻawale ai ka DNA kaula-lua i nā kaula hoʻokahi, e hoʻomākaukau ana no ka hoʻopaʻa mua ʻana i ka pōʻaiapuni o ka hopena.
2. Hoʻohui i ka DNA Template a me nā Primers: Ma hope o ka denaturation, e hoʻohaʻahaʻa ʻia ka mahana ma kahi o 55 ° C, e ʻae ana i nā primers e hoʻopaʻa i nā kaʻina hoʻohui ma ka DNA template kaula hoʻokahi.
3. Hoʻonui ʻia o nā Primers: Ma ka 72°C, e hoʻonui ʻia ka DNA template-primer complex me ke kōkua o DNA polymerase (e like me Taq DNA polymerase), me ka hoʻohana ʻana i nā dNTP ma ke ʻano he substrate e synthesize i kahi kaula hoʻohui hou e pili ana i ka DNA template.
Ma ka hana hou ʻana i nā kaʻina hana denaturation, annealing, a me ka hoʻonui ʻana, ʻoi aku ka nui o nā "semi-conservative replication strands" i hiki ke lilo i kumu hoʻohālike no nā pōʻai hope. ʻO kēlā me kēia pōʻaiapuni e pili ana i 2 a 4 mau minuke, e ʻae ana i ka gene i hoʻopaʻa ʻia e hoʻonui ʻia miliona mau manawa i loko o 2 a 3 mau hola.
ʻO Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he loea i ka hana ʻana i nā mea hana electrophoresis no nā makahiki he 50 me kā mākou hui loea loea a me ke kikowaena R&D. Loaʻa iā mākou ka laina hana hilinaʻi a piha mai ka hoʻolālā ʻana i ka nānā ʻana, a me ka hale kūʻai, a me ke kākoʻo kūʻai aku. ʻO kā mākou huahana nui ʻo Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Hoʻolako pū mākou i nā mea hana lab e like me ka mea PCR, vortex mixer a me ka centrifuge no ka hale hana.
Inā loaʻa iā ʻoe kahi hoʻolālā kūʻai no kā mākou huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou. Hiki iā ʻoe ke hoʻouna mai iā mākou i ka leka uila[palapala leka uila]a i ʻole[palapala leka uila], a i ʻole e kelepona iā mākou ma +86 15810650221 a i ʻole e hoʻohui iā Whatsapp +86 15810650221, a i ʻole Wechat: 15810650221.
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Ka manawa hoʻouna: Sep-24-2024