Ua pilikia paha ʻoe i ka hoʻomākaukau ʻana i ka gel agarose?E hahai maikā mākou ʻenehana lab i ka hoʻomākaukau ʻana i ka gel.
ʻO ke kaʻina hana hoʻomākaukau o ka agarose gel e pili ana i kēia mau hana:
Ke kaupaona ʻana i ka pauda Agarose
E kaupaona i ka nui i makemake ʻia o ka pauda agarose e like me ka manaʻo i makemake ʻia no kāu hoʻokolohua. Loaʻa ka nui o ka agarose maʻamau mai 0.5% a 3%. Hoʻohana ʻia nā haʻahaʻa kiʻekiʻe no ka hoʻokaʻawale ʻana i nā molekala DNA liʻiliʻi, aʻo nā haʻahaʻa haʻahaʻa no nā molekole nui.
Hoʻomākaukau ʻana i ka hoʻonā hoʻopaʻa
E hoʻohui i ka pauka agarose i kahi pale electrophoresis kūpono, e like me 1 × TAE a i ʻole 1 × TBE. Pono ka nui o ka buffer e like me ka nui o ka gel e pono ai no kāu hoʻokolohua.
Ka hoopau ana i ka Agarose
E puhi i ka agarose a me ka hui paʻa a hiki i ka pau ʻana o ka agarose. Hiki ke hana i kēia me ka microwave a i ʻole ka pā wela. E hoʻoulu i ka hopena i mea e pale ai i ka paila. Pono e akaka ka solution agarose me ka ʻike ʻole ʻia.
Hoʻomaʻalili i ka Haʻina Agarose
E ʻae i ka solution agarose i hoʻomehana ʻia e maʻalili ma kahi o 50-60°C. E hoʻoulu i ka hoʻonā i ka wā o ka hoʻoluʻu e pale i ka paʻa mua.
Hoʻohui ʻia ka ʻakika Nucleic Acid (Koho)
Inā makemake ʻoe e nānā i ka DNA a i ʻole RNA i loko o ka gel, hiki iā ʻoe ke hoʻohui i kahi stain nucleic acid, e like me GelRed a i ʻole ethidium bromide, i kēia manawa. I ka lawelawe ʻana i kēia mau ʻili, e hoʻokomo i nā mīkina lima a e akahele, no ka mea hiki ke make.
Ka hoolei ana i ka Gel
E ninini i ka ʻoluʻolu agarose i hoʻomaʻamaʻa ʻia i loko o kahi pahu electrophoresis gel i hoʻomākaukau ʻia. E hoʻokomo i kahi lehu no ka hana ʻana i nā pūnāwai laʻana, e hōʻoia i ka paʻa ʻana o ka lehu a ua puʻunaue like ʻia ka hopena i loko o ka poʻi.
Hoʻopaʻa Gel
E ʻae i ka gel e paʻa i ka lumi wela, ʻo ia ka mea maʻamau i 20-30 mau minuke ma muli o ka ʻike a me ka mānoanoa o ka gel.
Rka hoʻoneʻe ʻana i ka Comb
Ke paʻa pono ka gel, e wehe pono i ka lehu e hōʻike i nā luawai hāpana. E hoʻokomo i ka gel me ka poʻi i loko o ke keʻena electrophoresis a uhi iā ia me ka nui kūpono o ka electrophoresis buffer, e hōʻoia i ka piha piha ʻana o ka gel.
Hoʻomākaukau no ka Electrophoresis
Ma hope o ka mākaukau ʻana o ka gel, e hoʻouka i kāu mau laʻana i loko o nā pūnāwai, a hoʻomau i ka hoʻokolohua electrophoresis.
Inā loaʻa iā ʻoe kekahi pilikia me ka hoʻomākaukau ʻana i ka gel, e ʻoluʻolu e kelepona iā mākou. Loaʻa iā mākou nā loea lab ʻoihana e kamaʻilio me ʻoe.
Hauʻoli mākou e kaʻana like i kekahi nūhou nui: ʻo kā mākou tank electrophoresis horizontal DYCP-31DN kaulana i kēia manawa ke hoʻolaha nei, e leka uila iā mākou no ka ʻike hou aku i kēia manawa!
DYCP-31DN pahu pahu electrophoresis
ʻO Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he loea i ka hana ʻana i nā mea hana electrophoresis no nā makahiki he 50 me kā mākou hui loea loea a me ke kikowaena R&D. Loaʻa iā mākou ka laina hana hilinaʻi a piha mai ka hoʻolālā ʻana i ka nānā ʻana, a me ka hale kūʻai, a me ke kākoʻo kūʻai aku. ʻO kā mākou huahana nui ʻo Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Hoʻolako pū mākou i nā mea hana lab e like me ka mea PCR, vortex mixer a me ka centrifuge no ka hale hana.
Inā loaʻa iā ʻoe kahi hoʻolālā kūʻai no kā mākou huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou. Hiki iā ʻoe ke hoʻouna mai iā mākou i ka leka uila[palapala leka uila]a i ʻole[palapala leka uila], a i ʻole e kelepona iā mākou ma +86 15810650221 a i ʻole e hoʻohui iā Whatsapp +86 15810650221, a i ʻole Wechat: 15810650221.
E ʻoluʻolu e nānā i ka QR code e hoʻohui ma Whatsapp a i ʻole WeChat.
Ka manawa hoʻouna: Oct-15-2024