Ka hoʻomākaukau ʻana i kahi gel Agarose no ka Electrophoresis

Ka hoʻomākaukau ʻana o ka Agarose Gel no ka Electrophoresis

Nānā: E hoʻohana mau i nā mīkina lima hoʻopau!

Nā kuhikuhi ʻanuʻu

ʻO ke kaupaona ʻana o Agarose Powder:ue ka pepa kaupaona a me ke koena uila e ana i 0.3g o ka pauda agarose (e pili ana i kahi ʻōnaehana 30ml).

Ke hoʻomākaukau nei i ka TBST Buffer:pe hoʻihoʻi i 30ml o 1x TBST buffer i loko o kahi pahu Erlenmeyer 100ml.

Hoʻoheheʻe ʻia ʻo Agarose Powder:pkā mākou i ka pauka agarose i loko o ka pahu TBST a lulu maikaʻi.Hoʻokomo iā lākou i lokoi ka microwave a me ka wela (maamau no 50 kekona, uhi ʻia me ka alumini pepa) a hiki i ka hemo loa.

Hoʻoluʻu a hoʻohui i ka Nuclease:use mīkina lima e wehe i ka hui ʻana mai ka microwave a e hoʻomāalili iki i ka wai anuanu a mahana (ma kahi o 60 ° C). E hoʻohui i 2µl o ka nuclease (Eb substitute) oiai e lulu ana e hui pono.

Ka hoʻomākaukau ʻana i ka Mold Gel:

• Cwiwi a maloo kapahu gel a me ka mea hoolei gelo ka pahu electrophoresis.
•  Pkaula i ka gelpāi loko o ka pahu i loko a hoʻokomo i ka lehu ma kahi kūlana paʻa.
• Hoʻohui i ka solution gel agarose, hoʻoluʻu a hiki i 65 ° C, a ninini pono ma luna o kapahu geli kamea hoolele gel, hohola mālie a hiki i ka hana ʻana i kahi papa gel ma luna o ka pā aniani.
• E ʻae iā ia e noho ma ka lumi wela a paʻa loa ka gel.
• E wehe mālie i ka lehu a wehe i ka lipine.
• E kau i ka gelpāi loko o ka pahu electrophoresis.

Mea nui: E hōʻoia i ʻaʻohe ʻōhū ea ma kahi o nā niho lehu. Pono ka ʻili o ka wai e maʻemaʻe me ka ʻole o nā nalu.

Holo i ka Gel

Hoʻouka i ka Gel

Ma hope o ka paʻa ʻana o ka gel, e hoʻokomo iā ia i loko o ka pahu electrophoresis a ninini i ka pahu electrophoresis a hiki i ka hoʻoheheʻe ʻana o ka gel.

Hoʻomākaukau ʻana i nā Laʻana

• E lawe i ka marker a me ka hoʻouka ʻana i ka buffer mai ka friji.
• E hoʻohui i 6µl o ka hoʻouka paʻa i nā laʻana a hui maikaʻi.
• Ma ka hoʻohana ʻana i ka micropipette, e hoʻouka mālie i nā laʻana i loko o nā pūnāwai nui o ka gel (e makaʻala ʻaʻole e kī i ka gel a ʻaʻole e hoʻokaʻawale i ka leo holoʻokoʻa e pale aku i nā ʻōhū ea).
• E hoʻouka i ka māka i loko o kekahi o nā pūnāwai liʻiliʻi (e hoʻomanaʻo i kona kūlana).

Hoʻomaka Electrophoresis

• E uhi i ka pahu electrophoresis a hoʻomaka koke i ka electrophoresis ma hope o ka hoʻouka ʻana i ka gel.
• E hoʻonoho i ka voltage i 60-100V. E neʻe ana nā laʻana mai ka electrode maikaʻi ʻole (ʻeleʻele) i ka electrode maikaʻi (ʻulaʻula).
• Hoʻopau ka uila kiʻekiʻe i ka laulā hoʻokaʻawale kūpono o ka gel agarose.
• E ho'ōki i ka electrophoresis ke hiki aku ke kala uliuli bromophenol ma kahi o 1cm mai ka lihi lalo o ka papa gel.

Nānā i nā hopena

Ma hope o ka hoʻokaʻawale ʻana i nā kaula, hoʻōki i ka electrophoresis, lawe i waho i ka gel, a ʻike pono a kiʻi ʻia.

E hoʻohana i kahi ʻōnaehana kiʻi gel e kiʻi a nānā i nā kaula (e nānā inā loaʻa kekahi mau pū no ka marker a i ʻole nā ​​laʻana).

Ma hope o ka loaʻa ʻana o kāu palapala ʻāpana gel, e ʻimi i ka māka. Ma muli o ka marker, hiki iā ʻoe ke hoʻoholo i nā pūʻulu i hoʻopaʻa ʻia!

Ua hana ʻia ʻo Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) i nā huahana electrophoresis ma mua o 50 mau makahiki. Hōʻike mākou i kā mākou ʻōnaehana electrophoresis ākea ma aneʻi nogel agaroseelectrophoresis.E kohoʻōnaehana electrophoresis paemai Beijing liuyi no kāu hoʻokolohua gel electrophoresis e kōkua i kāu noiʻi ma ka ʻoihana biotechnology.

Hāʻawi mākou i nā hiʻohiʻona like ʻole o nā pahu electrophoresis horizontal no ka hoʻolei ʻana a me ka holo liʻiliʻiagarosegels i ka nuiagarosenā gels

Alakaʻi Koho no ka Unit Electrophoresis Gel Immersed Horizontal

ʻO Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he loea i ka hana ʻana i nā mea hana electrophoresis no nā makahiki he 50 me kā mākou hui loea loea a me ke kikowaena R&D. Loaʻa iā mākou ka laina hana hilinaʻi a piha mai ka hoʻolālā ʻana i ka nānā ʻana, a me ka hale kūʻai, a me ke kākoʻo kūʻai aku. ʻO kā mākou huahana nui ʻo Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Hoʻolako pū mākou i nā mea hana lab e like me ka mea PCR, vortex mixer a me ka centrifuge no ka hale hana.

Inā loaʻa iā ʻoe kahi hoʻolālā kūʻai no kā mākou huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou. Hiki iā ʻoe ke hoʻouna mai iā mākou i ka leka uila[palapala leka uila]a i ʻole[palapala leka uila], a i ʻole e kelepona iā mākou ma +86 15810650221 a i ʻole e hoʻohui iā Whatsapp +86 15810650221, a i ʻole Wechat: 15810650221.

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