ʻO ka hana ʻana o ka Polymerase Chain Reaction (PCR) a me ka Gel Electrophoresis: Nā ʻenehana koʻikoʻi i ka molecular biology.

Ma ke kahua o ka molecular biology, ua puka mai ka Polymerase Chain Reaction (PCR) a me Gel Electrophoresis ma ke ʻano he ʻenehana pōhaku kihi e hoʻomaʻamaʻa i ke aʻo ʻana a me ka hoʻoponopono ʻana i ka DNA. ʻAʻole pili wale kēia mau ʻano i ka noiʻi akā loaʻa nā noi ākea i ka diagnostics, forensic science, a me ka biotechnology.

Kiʻi mai ka pūnaewele biology4alevel

ʻO ka PCR kahi ʻenehana kipi i hoʻomohala ʻia e Kary Mullis i ka makahiki 1983, e hiki ai i nā ʻepekema ke hoʻonui i kahi ʻāpana DNA kikoʻī. Hoʻomaka ke kaʻina hana me ka denaturation o ka DNA, kahi i wela ai ka DNA stranded ʻelua ma kahi o 94 ° C, e hoʻokaʻawale ai i ʻelua mau kaula hoʻokahi. Hoʻopili ʻia kēia me ka hoʻopili ʻana, kahi e hoʻopaʻa ai nā primers—nā kaʻina pōkole o nā nucleotides-i nā kaʻina hoʻohui ma ka DNA kaula hoʻokahi ma kahi mahana haʻahaʻa (maʻamau ma kahi o 55°C). ʻO ka hope, hiki i ka manawa hoʻonui ma 72 ° C, kahi e hoʻohui ai ka enzyme DNA polymerase i kahi kaula hou o DNA ma o ka hoʻohui ʻana i nā nucleotides i nā primers. Hoʻopili hou ʻia kēia pōʻai 20-40 mau manawa, e alakaʻi ana i nā miliona o nā kope o ke kaʻina DNA target.

Pekina LIUYI PCR Mīkini

Ke hoʻonui ʻia ka DNA, hoʻohana ʻia ʻo Gel Electrophoresis e hoʻokaʻawale a nānā i nā huahana PCR. Hoʻopili kēia ʻenehana i ka neʻe ʻana o nā ʻāpana DNA ma o kahi matrix gel agarose ma lalo o ka mana o kahi kahua uila. Hoʻopiʻi maikaʻi ʻia nā molekala DNA ma muli o ko lākou iwi kuamoʻo phosphate, a neʻe lākou i ka electrode maikaʻi. Hana ka gel ma ke ʻano he kānana, e ʻae ana i nā ʻāpana DNA liʻiliʻi e neʻe wikiwiki ma mua o nā mea nui. ʻO ka hopena, ua hoʻokaʻawale ʻia nā ʻāpana DNA ma muli o ko lākou nui, me nā ʻāpana ʻokoʻa e ʻike ʻia ma lalo o ke kukui ultraviolet ma hope o ka hoʻopaʻa ʻia ʻana me kahi kala e like me ka ethidium bromide.

Pekina LIUYINā huahana Electrophoresis Gel

He ikaika ka hui pū ʻana o PCR a me Gel Electrophoresis i nā noi he nui. I nā diagnostics lapaʻau, hoʻohana ʻia ka PCR e ʻike i ka hiki ʻana o nā pathogens, genetic mutations, a i ʻole nā ​​ʻano DNA kikoʻī e pili ana i ka maʻi. A laila ʻae ʻo Gel Electrophoresis no ka ʻike a me ka hōʻoia ʻana o kēia mau ʻāpana DNA i hoʻonui ʻia. I ka ʻepekema forensic, he mea koʻikoʻi kēia mau ʻenehana no ka paʻi manamana lima DNA, kahi e kōkua ai lākou e hoʻohālikelike i nā hoʻohālike DNA mai nā hiʻohiʻona hewa me nā mea i manaʻo ʻia.

ʻO Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he loea i ka hana ʻana i nā mea hana electrophoresis no nā makahiki he 50 me kā mākou hui loea loea a me ke kikowaena R&D. Loaʻa iā mākou ka laina hana hilinaʻi a piha mai ka hoʻolālā ʻana i ka nānā ʻana, a me ka hale kūʻai, a me ke kākoʻo kūʻai aku. ʻO kā mākou huahana nui ʻo Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Hoʻolako pū mākou i nā mea hana lab e like me ka mea PCR, vortex mixer a me ka centrifuge no ka hale hana.

Pekina LIUYINā huahana Electrophoresis Gel

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