Polyacrylamide Gel Electrophoresis

Hoʻohana pinepine ʻia ka polyacrylamide i nā noi biology molecular e like me ke ʻano no ka electrophoresis o nā protein a me nā waikawa nucleic i kahi ʻenehana i kapa ʻia ʻo PAGE. He ʻano ʻano electrophoresis zone e ka synthetics gel i kapa ʻia ʻo polyacrylamide ma ke ʻano he kākoʻo. Ua kūkulu ʻia e S.Raymond lāua ʻo L.Weintraub i ka makahiki 1959, a laila hāpai ʻia a hoʻomohala ʻia e L.Ornstein lāua ʻo BJ Davis. Ua hoʻohana nui ʻia kēia ʻano hana ma hope o ka wehewehe ʻana a me ka hoʻololi hou ʻana e lākou i ka ʻenehana a me ka ʻenehana hoʻokolohua ma 1964.

Ma mua o ka hoʻohana ʻana i ka electrophoresis gel polyacrylamide, hoʻohana nui nā kānaka i ka electrophoresis pepa no ka zone EP. Akā ke hana nei ka pepa ma ke ʻano he ʻano, loaʻa wale ka hana o ka anti-convection, ʻaʻohe hopena maikaʻi ʻē aʻe. ʻOiai ʻo ka polyacrylamide gel ʻaʻole wale ka hana o ka anti-convection akā hiki ke komo pū i ka hoʻokaʻawale ʻana me ka ikaika. No ka mea ʻo ka polyacrylamide gel kahi ʻano o ka ʻupena ʻupena, ʻo ia ka polymerization a me ka hui cross-linking o acrylamide (Acr) a me N,N-methylenebis(acrylamide). Kapa ʻia ʻo Acrylamide he monomer, aʻo ka N, N-methylenebis i kapa ʻia he comonomer a i ʻole crosslinker. ʻO ka hana ʻana i kahi gel kahi hana o ka polymerization kemika. Hiki ke hoʻomalu ʻia ka nui o ka pore o kahi gel, no laila hiki ke hana ʻia ka gel me nā degere cross-linking like ʻole. Inā hoʻokokoke ka nui o ka pore i ka radius awelika o ka mole o ka hāpana, ʻo ke kūʻē o ka mole e hele i loko o ka pore gel e pili kokoke ana i ka nui a me ke ʻano o ka mole i ka wā o ka electrophoresis. No laila, hāʻawi ia i kahi mea hoʻokaʻawale hoʻololi no ka hoʻokaʻawale ʻana i kēlā mau mea me nā koina ʻupena like.

ʻElua mau ala maʻamau i hoʻohana ʻia no ka electrophoresis gel polyacrylamide, ʻo ka disc electrophoresis, a ʻo ka electrophoresis slab kekahi. Hoʻolālā kūikawā ʻia ka papa electrophoresis no ka hoʻokaʻawale ʻana i ka protein a me ka DNA, a ma ke ʻano maʻamau, ʻelua ʻano o ka papa electrophoresis, ʻo ia ka pahu electrophoresis horizontal a me ka pahu electrophoresis vertical. No ka protein, hoʻohana nā kānaka i ka pahu electrophoresis horizontal no IFF a me immunoelectrophoresis, i ʻole, hoʻohana nā kānaka i nā pahu electrophoresis vertical no nā protein.

ʻO Beijing Liuyi Biotechnology he mau ʻano pahu electrophoresis slab no PAGE, koe wale no ka nānā ʻana a me ka ʻike ʻana i nā ʻano protein e ka polyacrylamide gel electrophoresis, hiki ke hoʻohana ʻia no ke ana ʻana i ke kaumaha molekala, hoʻomaʻemaʻe i nā laʻana a me ka hoʻomākaukau ʻana i nā laʻana.

Lawe i ke kumu hoʻohālikeDYCZ-23Ae like me ka laʻana, ʻo ia kahi pahu electrophoresis maʻamau no Lab. E hoʻohana i ʻelua papa aniani e hana i ke keʻena gel e hana i ka gel, a laila e paʻi paʻa i nā pā aniani e pale i ka leaka gel. Aia ka mānoanoa o ka gel i ka mānoanoa o ka spacer. ʻO ka maʻamau, ʻo 1.5mm ka mānoanoa no ka hoʻokuʻu ʻana i ka wela, aDYCZ-23AHāʻawi pū kekahi i nā spacers mānoanoa 1.0mm no ka hoʻolei ʻana i ka gel 1.0mm. Ma waho o ka pahu electrophoresis, i mea e holo ai i ka electrophoresis, pono pū ka lako mana. Hāʻawi ʻo Beijing Liuyi Biotechnology i nā ʻano like ʻolelako mana electrophoresis. Mai ka uila kiʻekiʻe a hiki i ka haʻahaʻa haʻahaʻa, koho ʻoe i ke kumu hoʻohālike e like me ka noi.

Ua ʻoi aku ka nui o ka moʻolelo ʻo Beijing Liuyi ma mua o 50 mau makahiki ma Kina a hiki i ka hui ke hāʻawi i nā huahana kūpaʻa a kiʻekiʻe a puni ka honua. Ma o nā makahiki o ka hoʻomohala ʻana, kūpono ia i kāu koho!

Ke ʻimi nei mākou i nā hoa, ʻelua OEM electrophoresis tank a me nā mea hoʻolaha i hoʻokipa ʻia.

No ka ʻike hou aku e pili ana iā mākou, e ʻoluʻolu e kelepona mai iā mākou ma ka leka uila[palapala leka uila]a i ʻole[palapala leka uila].