ʻO ka Comet Assay (Single Cell Gel Electrophoresis, SCGE) kahi hana maʻalahi a wikiwiki hoʻi i hoʻohana mua ʻia e ʻike i ka pōʻino a me ka hoʻoponopono ʻana i ka DNA i loko o kēlā me kēia cell. ʻO ka inoa ʻo "Comet Assay" mai ke ʻano like me ka comet i ʻike ʻia i nā hopena: ʻo ka nucleus o ka cell e hana i ka "poʻo," aʻo ka neʻe ʻana o nā ʻāpana DNA i hōʻino ʻia, e hana ana i kahi "huelo" e like me ka comet.
Kumukānāwai
Hoʻokumu ʻia ke kumumanaʻo o ka Comet Assay i ka neʻe ʻana o nā ʻāpana DNA i kahi kahua uila. Noho ʻo DNA intact i loko o ka nucleus cell, ʻoiai ʻo DNA i pōʻino a ʻāpana ʻāpana e neʻe aku i ka anode, e hana ana i ka "huelo" o ka comet. ʻO ka lōʻihi a me ka ikaika o ka huelo e pili pono ana i ka nui o ka pōʻino DNA.
Kaʻina hana
- Hoʻomākaukau Cell: Hoʻohui ʻia nā cell e hoʻāʻo ʻia me ka agarose haʻahaʻa hoʻoheheʻe ʻia a hoʻolaha ʻia ma nā paheʻe microscope e hana i kahi papa like.
- Cell Lysis: Hoʻokomo ʻia nā paheʻe i loko o kahi solution lysis e wehe i ka membrane cell a me ka membrane nuklea, e hōʻike ana i ka DNA.
- Electrophoresis: Hoʻokomo ʻia nā paheʻe i loko o kahi keʻena electrophoresis ma lalo o nā kūlana alkaline a kū ʻole paha. Holo nā ʻāpana DNA i pōʻino i ka electrode maikaʻi ma lalo o ka mana o ke kahua uila.
- ʻaʻahu ʻana: Ma hope o ka electrophoresis, hoʻopaʻa ʻia nā paheʻe me kahi mea hoʻonani fluorescent (e laʻa, ethidium bromide) e ʻike i ka DNA.
- Hoʻomāliʻi kiʻekiʻe: Me ka hoʻohana ʻana i ka microscope fluorescence a i ʻole nā lako polokalamu kūikawā, e nānā ʻia nā ʻano kometa, a ua ana ʻia nā ʻāpana e like me ka lōʻihi o ka huelo a me ka ikaika.
Kiʻi mai biorender
ʻIkepili ʻikepili
ʻIke ʻia nā hopena mai ka Comet Assay e pili ana i nā ʻāpana koʻikoʻi:
- Ka lōʻihi o ka huelo: Hōʻike i ka mamao e neʻe ai ka DNA, e hōʻike ana i ka nui o ka pōʻino DNA.
- Maʻiʻo DNA huelo: ʻO ka pākēneka o DNA e neʻe ana i ka huelo, hoʻohana pinepine ʻia ma ke ʻano he ana nui o ka pōʻino DNA.
- Ka Manawa Huʻu Olive (OTM): Hoʻohui i ka lōʻihi o ka huelo a me ka ʻike DNA huelo e hāʻawi i kahi ana ʻoi aku ka nui o ka pōʻino DNA.
Nā noi
- Haʻawina Genotoxicity: Hoʻohana nui ʻia ka Comet Assay e loiloi i nā hopena o nā kemika, nā lāʻau lapaʻau, a me ka radiation ma ke cell DNA, e lilo ia i mea hana nui no ka hoʻāʻo ʻana i ka genotoxicity.
- Hoʻonaʻauao Kaiapuni: Kōkua ia i ka nānā ʻana i ka hopena o nā mea haumia kaiapuni i ka DNA o nā mea ola, e hāʻawi ana i nā ʻike i ka palekana kaiaola.
- Noiʻi Lapaʻau a me nā Kauka: Hoʻohana ʻia ka Comet Assay i ke aʻo ʻana i nā mīkini hoʻoponopono DNA, ka maʻi kanesa, a me nā maʻi e pili ana i ka DNA. He loiloi pū kekahi i ka hopena o nā lāʻau lapaʻau e like me ka radiotherapy a me ka chemotherapy ma DNA.
- ʻEpekema Meaʻai a Mahiʻai: Hoʻohana ʻia e loiloi i ka palekana o nā pesticides, nā mea hoʻohui meaʻai, a me nā mea ʻē aʻe, a no ke aʻo ʻana i kā lākou hopena ʻona i nā ʻano holoholona.
Nā pono
- Kiekie Kiekie: Hiki ke ʻike i nā pae haʻahaʻa o ka pōʻino DNA.
- Hana maʻalahi: He mea maʻalahi ka ʻenehana, kūpono ia no ka nānā ʻana i nā kiʻekiʻe.
- Hoʻohana ākea: Hiki ke hoʻohana ʻia i nā ʻano cell like ʻole, me nā pūnae holoholona a me nā mea kanu.
- Nā Luʻi Kuʻuna: ʻOiai e hāʻawi ana i ka ʻikepili qualitative e pili ana i ka pōʻino DNA, hilinaʻi nui ka nānā ʻana i nā lako polokalamu a me nā ʻenehana loiloi kiʻi.
- Kūlana hoʻokolohua: Hiki ke hoʻololi ʻia nā hopena e nā mea like me ka manawa electrophoresis a me ka pH, e koi ana i ka mālama pono ʻana i nā kūlana hoʻokolohua.
Nā palena
He mea waiwai nui ka Comet Assay i ka noiʻi biomedical, ka ʻepekema kaiapuni, a me ka hoʻomohala ʻana i ka lāʻau ma muli o kona maʻalahi a me ka ʻike kiʻekiʻe i ka ʻike ʻana i ka pōʻino a me ka hoʻoponopono DNA. Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology)hāʻawi i ke keʻena electrophoresis ākea no ka hoʻāʻo comet. Welina mai e launa pū me mākou e kūkākūkā e pili ana i kaʻIke Kometaprotocol.
ʻO Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he loea i ka hana ʻana i nā mea hana electrophoresis no nā makahiki he 50 me kā mākou hui loea loea a me ke kikowaena R&D. Loaʻa iā mākou ka laina hana hilinaʻi a piha mai ka hoʻolālā ʻana i ka nānā ʻana, a me ka hale kūʻai, a me ke kākoʻo kūʻai aku. ʻO kā mākou huahana nui ʻo Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Hoʻolako pū mākou i nā mea hana lab e like me ka mea PCR, vortex mixer a me ka centrifuge no ka hale hana.
Inā loaʻa iā ʻoe kahi hoʻolālā kūʻai no kā mākou huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou. Hiki iā ʻoe ke hoʻouna mai iā mākou i ka leka uila[palapala leka uila]a i ʻole[palapala leka uila], a i ʻole e kelepona iā mākou ma +86 15810650221 a i ʻole e hoʻohui iā Whatsapp +86 15810650221, a i ʻole Wechat: 15810650221.
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Ka manawa hoʻouna: Sep-20-2024