Hoʻomākaukau Laʻana a meKe hoʻouka nei
Ma muli o ka hoʻohana ʻana i kahi ʻōnaehana pale hoʻomau me ka ʻole o ka hoʻopaʻa ʻana i ka gel i ka hapa nui o nā hihia, pono e loaʻa i nā laʻana kahi ʻano kūpono a me kahi leo liʻiliʻi. E hoʻohana i kahipipettee hoʻohui mālie i ka hāpana, me 5-10 μg no ka pūnāwai, e pale i ka emi nui o ka hoʻonā. I ka manawahoʻouka laʻana, pono e pio ka mana. Pono e loaʻa i ka hāpana kekahi mea pena hōʻailona (0.025% bromophenol blue a ʻalani paha) a me ka sucrose (10-15%) a i ʻole glycerol (5-10%) e hoʻonui ai i kona mānoanoa, hoʻopaʻa i ka hāpana, a pale i ka laha. Eia nō naʻe, i kekahi manawa, hiki i ka sucrose a i ʻole glycerol ke hoʻoulu i nā kaula U-like i nā hopena electrophoresis, hiki ke pale ʻia me ka hoʻohana ʻana i ka 2.5% Ficoll (polyvinylpyrrolidone).
Electrophoresis
ʻO ka volta no ka electrophoresis he 5-15 V/cm, ma kahi o 10 V/cm. No ka hoʻokaʻawale ʻana o nā molelaki nui, pono e emi ka volta, ʻaʻole maʻamau ma mua o 5 V / cm.
ʻaʻahu ʻana
Hoʻohana pinepine ʻia ka ethidium bromide (EB) dye fluorescent no ka ʻili ʻana e nānā i nā kaula DNA i ka gel agarose. Hiki i ka EB ke hoʻokomo ma waena o nā paʻa kumu o nā molekala DNA, e hoʻopili ai ʻo EB me DNA. Hoʻololi ʻia ka lawe ʻana o ke kukui ultraviolet 260 nm e DNA i EB, a ʻo ka EB i hoʻopaʻa ʻia e hoʻokuʻu i ka fluorescence ma 590 nm i ka ʻāpana ʻulaʻula-ʻalani o ke kiko kukui ʻike. ʻO ka hoʻopaʻa ʻana i ka gel i loko o kahi solution 1 mmol/L MgSO4 no ka hola 1 hiki ke hōʻemi i ka fluorescence hope i hoʻokumu ʻia e ka EB unbound, e hoʻomaʻamaʻa i ka ʻike ʻana i nā helu liʻiliʻi o DNA.
He nui nā pōmaikaʻi o ka dye EB: maʻalahi ke hoʻohana, ʻaʻole e uhaʻi i nā waikawa nucleic, kiʻekiʻe ka naʻau, a hiki ke hoʻopaʻa i ka DNA a me RNA. Hiki ke hoʻohui ʻia ʻo EB i ka laʻana a nānā ʻia me ka hoʻohana ʻana o UV i kēlā me kēia manawa. Ma hope o ka hoʻopaʻa ʻana, hiki ke hoʻoneʻe ʻia ka EB ma ka unuhi ʻana me ka n-butanol.
Eia nō naʻe, he mutagen ikaika ka dye EB, a pono e mālama ʻia ka mālama ʻana, e like me ke kau ʻana i nā mīkina lima polyethylene. ʻO ka ʻalani ʻalani kekahi mea hoʻohana maʻamau no ka mea hiki iā ia ke hoʻokaʻawale i waena o nā ʻakika nucleic stranded-stranded a me nā ʻakika nucleic pālua (DNA, RNA). Hōʻike ia i ka ʻōmaʻomaʻo ʻōmaʻomaʻo (530 nm) no nā ʻakika nucleic pae ʻelua a me ka fluorescence ʻulaʻula (640 nm) no nā ʻakika nucleic single-stranded. Eia kekahi, hiki ke hoʻohana ʻia nā mea pena e like me ka methylene blue, methylene green, a me ka quinoline B no ka hoʻopaʻa ʻana.
ʻO Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) he loea i ka hana ʻana i nā mea hana electrophoresis no nā makahiki he 50 me kā mākou hui loea loea a me ke kikowaena R&D. Loaʻa iā mākou ka laina hana hilinaʻi a piha mai ka hoʻolālā ʻana i ka nānā ʻana, a me ka hale kūʻai, a me ke kākoʻo kūʻai aku. ʻO kā mākou huahana nui ʻo Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Ke ʻimi nei mākou i nā hoa, ʻelua OEM electrophoresis tank a me nā mea hoʻolaha i hoʻokipa ʻia.
Inā loaʻa iā ʻoe kahi hoʻolālā kūʻai no kā mākou huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou. Hiki iā ʻoe ke hoʻouna mai iā mākou i ka leka uila[palapala leka uila]a i ʻole[palapala leka uila], a i ʻole e kelepona iā mākou ma +86 15810650221 a i ʻole e hoʻohui iā Whatsapp +86 15810650221, a i ʻole Wechat: 15810650221.
E ʻoluʻolu e nānā i ka QR code e hoʻohui ma Whatsapp a i ʻole WeChat.
Ka manawa hoʻouna: Dec-20-2023