ʻO Agarose Gel Electrophoresis o RNA

ʻO kahi haʻawina hou mai RNA

I kēia mau lā, ʻike ʻia kahi noiʻi i nā ʻano genetice ho'ēmi i nā pae hoʻoponopono o ka RNA kaulua ʻelua e pili ana me nā kūlana autoimmune a me ka immune-mediated.

Hiki i nā molekala RNA ke hoʻololi. No ka laʻana, hiki ke hoʻokomo ʻia nā nucleotides, holoi ʻia, a hoʻololi paha. ʻO kekahi o nā hoʻoponopono maʻamau, kahi i hōʻike ʻia e nā noiʻi hou he kuleana koʻikoʻi i ka hoʻomaka ʻana o ka maʻi inflammatory, ʻo ia ka hoʻololi ʻana o ka nucleotide adenosine i inosine i loko o kahi RNA pālua. He haʻawinauapaʻi ʻiaon Hōʻike ʻo ʻAukake 3 ma Nature e pili ana nā ʻano ʻano genetic e hoʻoheheʻe i kēia hoʻololi kikoʻī me ka piʻi nui ʻana o nā maʻi maʻi autoimmune a me ka immune-mediated inflammatory e like me psoriasis, ka maʻi ʻeha o ka ʻōpū, a me ka maʻi diabetes type 1. Manaʻo nā mea kākau e kuhi hewa paha kahi protein sensor i kēia mau RNA liʻiliʻi i hoʻoponopono ʻia no nā molekole haole, e hoʻoulu ai i kahi pane inflammatory.

Ua manaʻo ka mea olaola molekula'he holomua nui loa, a loaʻaʻo ia ka mea "hoʻokūpaʻa maoli" i ka manaʻo nui o ka hoʻoponopono RNA no nā maʻi inflammatory, ke hoʻohālikelike ʻia me ka hōʻike gene a i ʻole ka splicing.

He noiʻi nui maoli nō. A ma ke ʻano he kūʻai huahana electrophoresis, makemake wau e pili ana i ke ʻano o RNA, a pehea e nānā ai nā ʻepekema i ka RNA e ka electrophoresis? He ʻike ʻoihana maoli a ʻike ʻole ia iaʻu akā naʻe he leʻaleʻa ke ʻimi.

He aha ka RNA?

ʻO RNA, pōkole no Ribonucleic acid, he waikawa nucleic i loaʻa i loko o nā pūnaewele ola a pau. ʻAʻole like me DNA, ʻo ka RNA ka mea maʻamau maʻamau. ʻO kāna hana nui ʻo ia ka hana ma ke ʻano he ʻelele e lawe ana i nā ʻōlelo aʻoaʻo mai DNA no ka hoʻomalu ʻana i ka synthesis o nā protein, ʻoiai ma kekahi mau maʻi maʻi ʻo RNA ma mua o ka DNA e lawe i ka ʻike genetic.

ʻO Agarose Gel Electrophoresis o RNA

Hiki i nā kānakae nānā i ka maikaʻi o ka RNA ma ka electrophoresis gel.

Beijing Liuyi Biotechnology Co.Ltd, amea hana loea e kālele ana i nā huahana electrophoresis ma luna o 50 makahiki, hāʻawi i kahi ʻano o nā pahu electrophoresis (chambers/cells) no ka electrophoresis gel, e like me nā pahu electrophoresis horizontal (chambers/cells) no ka electrophoresis gel agarose, a me nā pahu electrophoresis vertical (chambers/cells) no protein gel electrophoresis. Eia nō naʻe, hāʻawi ia i nā ʻano like ʻole o ka mana electrophoresis, ka mea nānā pahu pahu pouli a me ka transilluminator UV. Hiki iā ʻoe ke koho iā Beijing Liuyi's electrophoresis tanks (chambers/cells) e ana i ka molecular weight of RNA a e hoʻokaʻawale i ka RNA o nā ʻano nui like ʻole no kāu lab.

Ma ʻaneʻi e hoʻolauna mākou i hoʻokahi ʻano o ka pahu electrophoresis horizontal (chamber/cell) model DYCP-31DN mai kā mākou huahana 31 series.

ʻO DYCP-31DN kahi pahu electrophoresis ākea liʻiliʻi (keʻena / cell) no ke ana ʻana i ke kaumaha molekala o RNA a i ʻole DNA a hoʻokaʻawale iā lākou.