Gene Electroporator GP-3000
Nā kikoʻī
| Hoʻohālike | GP-3000 |
| Pulse Form | Ka Pohō Pākuʻi a me ka Hawewe huinahā |
| Puke uila kiʻekiʻe | 401-3000V |
| Puka uila haʻahaʻa | 50-400V |
| Kiʻekiʻe voltage capacitor | 10-60μF i nā ʻanuʻu 1μF (10μF, 25μF, 35μF, 50μF, 60μF manaʻo ʻia) |
| Kapili uila haʻahaʻa | 25-1575μF i nā ʻanuʻu 1μF (manaʻo ʻia nā ʻanuʻu 25μF) |
| Palena pale like | 100Ω-1650Ω ma 1Ω mau ʻanuʻu (50Ω manaʻo ʻia) |
| Lako ikehu | 100-240VAC50/60HZ |
| ʻōnaehana hana | Ka mana microcomputer |
| Mau manawa | me ka manawa RC mau, hiki ke hoʻololi |
| ʻupena paona | 4.5kg |
| Nā ʻāpana pūʻolo | 58x36x25cm |
wehewehe
He ʻano koʻikoʻi ka electroporation cell no ka hoʻokomo ʻana i nā macromolecules exogenous e like me DNA, RNA, siRNA, proteins, a me nā molekole liʻiliʻi i loko o nā membrane cell.
Ma lalo o ka mana o ke kahua uila ikaika no kekahi manawa, loaʻa i ka membrane cell i loko o ka hopena i kahi permeability. Hoʻokomo ʻia nā mea exogenous i hoʻopaʻa ʻia i ka membrane cell ma ke ʻano like me ka electrophoresis. Ma muli o ke kūpaʻa kiʻekiʻe o ka phospholipid bilayer o ka membrane cell, ʻo nā voltage bipolar i hana ʻia e ke kahua uila o waho e halihali ʻia e ka membrane cell, a hiki ke mālama ʻole ʻia ka uila i hāʻawi ʻia i ka cytoplasm, me ka aneane ʻaʻohe o kēia manawa i ka cytoplasm. no laila e hoʻoholo ai i ka liʻiliʻi liʻiliʻi i loko o ke ʻano maʻamau o ke kaʻina hana electrophoresis.
Palapala noi
Hiki ke hoʻohana ʻia no ka electroporation e hoʻololi i ka DNA i loko o nā pūnaewele mākaukau, nā mea kanu a me nā mea holoholona, a me nā mea hū. E like me ka electroporation o ka bacteria, yeast, a me nā microorganisms ʻē aʻe, ka neʻe ʻana o nā cell mammalian, a me ka neʻe ʻana o nā ʻiʻo o nā mea kanu a me nā protoplasts, ka hybridization cell a me ka hoʻohui ʻana o nā genes, ka hoʻokomo ʻana i nā genes marker no ka lepili a me nā kumu hōʻike, hoʻokomo i nā lāʻau lapaʻau, nā protein, nā antibodies, a me nā molekala ʻē aʻe e aʻo i ke ʻano a me ka hana o nā cell.
Hiʻona
• 'O ke kūlana ki'eki'e: ka manawa ho'ololi pōkole, ke ki'eki'e o ka ho'ololi'ana, ka hana hou;
• Waihona akamai: hiki ke mālama i nā palena hoʻokolohua, kūpono i nā mea hoʻohana e hana;
• Ka mana pololei: ka hoʻokuʻu ʻana i ka pulse microprocessor;Ø
• He hiʻohiʻona nani: hoʻolālā hoʻohui o ka mīkini holoʻokoʻa, hōʻike intuitive, hana maʻalahi.
FAQ
Nīnau: He aha ka Gene Electroporator?
A: ʻO ka Gene Electroporator kahi mea hana i hoʻohana ʻia no ka hoʻokomo ʻana i nā mea genetic exogenous, e like me DNA, RNA, a me nā protein, i loko o nā cell ma o ke kaʻina hana electroporation.
Nīnau: He aha nā ʻano o nā cell e hiki ke hoʻopaʻa ʻia me kahi Gene Electroporator?
A: Hiki ke hoʻohana ʻia kahi Gene Electroporator no ka hoʻokomo ʻana i nā mea genetic i loko o nā ʻano cell like ʻole me ka bacteria, yeast, cell plant, mammalian cell, a me nā microorganism ʻē aʻe.
Nīnau: He aha nā noi nui o kahi Gene Electroporator?
A:
• Electroporation o bacteria, yeast, a me nā microorganisms ʻē aʻe: No ka hoʻololi genetic a me nā haʻawina hana gene.
• Ka hoʻololi ʻana o nā pūnaewele mammalian, nā ʻiʻo mea kanu, a me nā protoplasts: No ka nānā ʻana i ka hōʻike ʻano gene, nā genomics hana, a me ka ʻenekinia genetic.
• ʻO ka hoʻohui ʻana o ke kelepona a me ka hoʻohui ʻana i nā genes: No ka hoʻokumu ʻana i nā cell hybrid a me ka hoʻokomo ʻana i nā genes fusion.
• Hoʻomaka i nā genes marker: No ka hōʻailona ʻana a me ka nānā ʻana i ka hōʻike gene i loko o nā cell.
• Hoʻokomo i nā lāʻau lapaʻau, nā protein, a me nā antibodies: No ka noiʻi ʻana i ke ʻano o ka cell a me ka hana, ka lawe ʻana i ka lāʻau, a me nā haʻawina pili pūmua.
Nīnau: Pehea e hana ai kahi Gene Electroporator?
A: Hoʻohana ka Gene Electroporator i kahi puʻupuʻu uila pōkole a kiʻekiʻe e hana i nā pores pōkole i loko o ka membrane cell, e ʻae ana i nā molekole exogenous e komo i loko o ke kelepona. Hoʻopaʻa hou ka membrane cell ma hope o ka pulse uila, e hoʻopaʻa ana i nā molekala i hoʻokomo ʻia i loko o ke kelepona.
Nīnau: He aha nā mea maikaʻi o ka hoʻohana ʻana i kahi Gene Electroporator?
A: Kiʻekiʻe ka hana hou a me ka pono, maʻalahi o ka hana: ʻO ke kaʻina hana maʻalahi a me ka wikiwiki, ka mana quantitative, ʻaʻohe genotoxicity: ʻO ka liʻiliʻi o ka pōʻino i nā mea genetic o ke kelepona.
Nīnau: Hiki ke hoʻohana ʻia kahi Gene Electroporator no nā ʻano hoʻokolohua āpau?
A: ʻOiai ʻo ka Gene Electroporator he mea maʻalahi, hiki ke hoʻololi ʻia kona pono ma muli o ke ʻano kelepona a me ka mea genetic i hoʻokomo ʻia. He mea nui e hoʻonui i nā kūlana no kēlā me kēia hoʻokolohua kikoʻī.
Nīnau: He aha ka mālama kūikawā e pono ai ma hope o ka hoʻokomo?
A: Hiki i ka mālama ma hope o ka hoʻomaka ʻana ke komo i ka hoʻoulu ʻana i nā cell i kahi ala hoʻōla e kōkua iā lākou e hoʻoponopono a hoʻomau i nā hana maʻamau. Hiki ke ʻokoʻa nā kikoʻī ma muli o ke ʻano kelepona a me ka hoʻokolohua.
Nīnau: Aia kekahi pilikia palekana me ka hoʻohana ʻana i kahi Gene Electroporator?
A: Pono e hahai ʻia nā hana palekana laboratory maʻamau. Hoʻohana ʻo Gene Electroporator i ka uila kiʻekiʻe, no laila pono e mālama pono i nā kaʻina hana a me ka palekana i mea e pale ai i nā pōʻino uila.
