Kelepona Electrophoresis no SDS-PAGE a me Western Blot
Hōʻike
| Ana (L×W×H) | 140×100×150mm |
| Nui o ka Gel (L×W) | 75×83mm |
| Comb | 10 luawai a me 15 luawai |
| Mānoanoa Hui | 1.0mm a me 1.5mm (Maʻamau) 0.75mm (Koho) |
| Ka helu o na laana | 20-30 |
| Volume Hoʻopaʻa | 400ml |
| Kaumaha | 1 kg |
wehewehe
ʻO DYCZ-24DN kahi pūnaewele electrophoresis vertical (tank / chamber) no SDS-PAGE, Native PAGE etc. Protein electrophoresis. Hiki i kēia kelepona ke hoʻolei a holo i ka gel ma kahi hoʻokahi. He maʻalahi a kūʻokoʻa ka mea maʻalahi a maʻalahi hoʻi e hoʻouka i nā laʻana. Hana ʻia ka pahu i nā mea polycarbonate kiʻekiʻe, ʻoi loa ka lōʻihi a me ka ʻike. He mea maʻalahi ka nānā ʻana i ka gel i ka wā e hana ai ka hoʻokolohua. Loaʻa i ka DYCZ-24DN nā electrodes hiki ke wehe i maʻalahi no ka mālama. Hana ʻia nā electrodes e ka platinum maʻemaʻe (≥99.95%) he electrolysis-corrosion a kū i ka wela kiʻekiʻe.
Ma hope o ka electrophoresis gel, e like me ke koi hoʻokolohua, i kekahi manawa, pono ka mea hoʻokolohua e hoʻololi i ka gel i kahi kākoʻo paʻa no ka nānā hou aku. Kapa ʻia ʻo ia ka hoʻokolohua blotting, ʻo ia ke ʻano o ka hoʻoili ʻana i nā protein, DNA a i ʻole RNA ma luna o kahi mea lawe. Hana ʻia ia ma hope o ka electrophoresis gel, e hoʻoneʻe i nā molekala mai ka gel i ka membrane blotting. Ma hope o ka holoi ʻana, ʻike ʻia nā protein i hoʻololi ʻia, DNA a i ʻole RNA e ke kala kala (no ka laʻana, ke kala kala o nā protein), ka ʻike autoradiographic o nā molekole radiolabelled (hana ʻia ma mua o ka blot), a i ʻole ka hōʻailona kikoʻī o kekahi mau protein a i ʻole nā waikawa nucleic. Hana ʻia ka hope me nā antibodies a i ʻole hybridization probes e hoʻopaʻa wale i kekahi mau mole o ka blot a loaʻa kahi enzyme i hui pū ʻia me lākou. Ma hope o ka holoi pono ʻana, ʻike ʻia kēia hana enzymatic (a pēlā, nā molekala a mākou e ʻimi ai i ka blot) e ka incubation me ka reactive kūpono, e hāʻawi ana i kahi waiho waihoʻoluʻu ma ka blot a i ʻole ka hopena chemiluminescent i hoʻopaʻa inoa ʻia e ke kiʻi kiʻi.
No ka lako mana no kēia pūnaʻi electrophoresis gel kūpaʻa, manaʻo mākou i kekahi o ka mana electrophoresis hoʻokele manawa i ke kumu hoʻohālike DYY-6C.
Palapala noi
No SDS-PAGE, Native PAGE electrophoresis a me ka hoʻololi ʻana i ka mole protein mai ka gel a i ka membrane.
Hiʻona
ʻO ka DYCZ-24DN mini vertical gel electrophoresis cell no SDS-PAGE, Native PAGE electrophoresis loaʻa nā hiʻohiʻona penei:
•Hana ʻia me ka polycarbonate transparent kiʻekiʻe, nani a paʻa, maʻalahi no ka nānā ʻana;
• Me ka hoʻolei gel i ke kūlana mua, hiki ke hoʻolei a holo i ka gel ma kahi hoʻokahi, maʻalahi a maʻalahi e hana i nā gels, a mālama i kou manawa waiwai;
• Hiki ke ho'opololei pa'a ka lumi gel;
• Hoʻopili ʻia ka pahu pahu pahu i hoʻolako ʻia i nā electrodes platinum maʻemaʻe;
• maʻalahi a maʻalahi e hoʻohui i nā laʻana;
•Hiki iā rhoʻokahi gel a ʻelua paha gel i ka manawa like;
• E mālama i ka hopena pale;
• Hoʻolālā kūikawā o ka pahu e pale i ka pale a me ka leaka gel;
•Hiki ke hoʻoneʻe i nā electrodes, maʻalahi e mālama a hoʻomaʻemaʻe;
• Ho'opi 'akomi ke wehe 'ia ka po'i;
ʻO ka module electrode, i kapa ʻia ʻo ke kino kākoʻo no ka hoʻololi ʻana a i ʻole ka hui electrode he ʻāpana nui no ka ʻōnaehana blotting DYCZ-40D. Loaʻa iā ia nā ʻāpana ʻulaʻula a me ka ʻeleʻele a me nā electrodes ʻulaʻula a ʻeleʻele e hōʻoia i ka hoʻonohonoho pono ʻana o ka gel i ka wā o ka hoʻololi ʻana, a me kahi hoʻolālā kūpono e hoʻomaʻamaʻa i ka hoʻokomo ʻana a me ka wehe ʻana i nā cassette paʻa gel mai ke kino kākoʻo no ka hoʻoili ʻana (electrode assembly).
